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DNS is widely used in manual determinations; however, its requirement of high temperatures (100°C) for colour development and the presence of phenol in the DNS reagent make this method difficult to adapt to standard liquid handling equipment.
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Automated calling (in which PCR fragment sizes and allele assignment are automatically determined) or manual determination of genotyping data (number of repeats for each loci) was entered into Microsoft Excel (Redmond, WA, USA) tables and imported for further analysis into BioNumerics software (Applied Maths, St Martens, Belgium).
The simplest mechanisms will be manual determination and confirmation of authenticity and trust level.
For now I'll stick with NetNewsWire and hope that my own manual determination of relevance doesn't leave me missing out on too much important news.
The manual determination of chemical reaction networks (CRN) and reaction rate equations is cumbersome and becomes workload prohibitive for large systems.
Penetrometer ECa data also provided efficient and accurate (r2 = 0.92) estimation of calibration-point topsoil depth as an alternative to soil coring and manual determination.
An example of manual determination of genotyping is shown in Figure 2B.
In manual determination of the genotypes of the S-WGA products, we arbitrarily defined the following four types of thresholds, based on the fluorescence intensities (FI) of the control samples.
To avoid the manual
However, manual determination of PP is difficult, particularly in the tachypneic patient and in noisy clinical environments[ 18- 21].
The results obtained using the automated platform showed similar differences in saccharification to those obtained by manual determination.
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