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Detailed information about data collection and manual curation has been included in the additional file 1.
In recent years, interest in the issue of assisting manual curation has dramatically increased (26).
Manual curation has long been used for extracting key information found within the primary literature for input into biological databases.
The growing burden on manual curation has spurred the development of text mining tools for biological entities and relations (including protein interactions).
The sources of phenotypic data used in the present study are metadatabases in which uniformity criteria and manual curation has been imposed on assembled records.
Although manual curation has been assumed to be highly accurate, we are aware of only one previous study of biocuration accuracy.
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This prevents domains from being incorrectly matched to the first 7 SCOP classes, in cases where manual curation had put the domain in classes 8 and above.
Our bioinformatics searches and exhaustive manual curation have yielded what we are confident should be an extremely comprehensive set of eLRR proteins across the four species examined.
Our initial DDF metadata for over 220 resources was captured as part of a detailed MRB questionnaire sent to each resource, and active manual curation had to be used to fill in the gaps in responses.
As of early 2013, Kinannote, or a combination of Kinannote and manual curation, have been used to annotate the kinomes of 36 genomes produced by the Broad Institute (Supplementary Table S2).
However, because of their inconsistency in translation to GO terms and relations, manual curations have encountered many difficulties (Ashburner et al., 2001).
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