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The genomes of these six phages were sequenced to completion by 454 pyrosequencing followed by manual closure, representing a total combined DNA sequence of 1.33 Mb.
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They focused mainly on the two most commonly practiced interventions: stapler versus manual oversewn closure of the pancreatic remnant [ 27– 29].
The results are heterogeneous and often contradictory: several authors have shown lower fistula rates in manual oversewn closure compared to stapler transection [ 6, 14– 16], while others favored stapler [ 17– 17].
Some had a dye test and some (approximately one-third of cases) did not; notably, dye test is considered a standard step in FIGO's training manual, to confirm closure, integrity of the repair and also that there is no additional fistula that has been overlooked [ 5].
A self-help manual, If You Want Closure in Your Relationship, Start With Your Legs, won the Diagram prize for Oddest Book Title of the Year.
Gap closure and manual inspection and editing were performed using the SeqMan software (v.5.0.3).
The surgical techniques ranged from the standard techniques used most commonly (stapler closure and manual hand-sewn closure [ 27, 37]) to techniques used less frequently (ultrasonic dissection, closure with jejunal seromuscular patch, polypropylene mesh [ 36, 37, 39]).
Bp strains 1106a, 1710b, and 668 were sequenced with full closure and manual annotation, whereas 1655, 406e, S13, and Pasteur 52237 were sequenced without closure and annotated automatically to 8× coverage.
It is not clear whether the "close command" occurs because of a re-closure signal, manual close signal or a combination of the two.
Advantages of vascular closure device over manual compression include patient comfort, early mobilisation and discharge, avoidance of interruption of anticoagulation, avoidance of local compression and its sequelae and less time constraint on staff.
Key features of the LAREDO sampler include the novel application (in this environment) of a torsion bar to generate the energy required to seal the sample chambers, independent and continuous temperature records in two of the three sample chambers, and remote (electronic) or manual (by submersible) modes of closure.
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