Sentence examples for manual chip from inspiring English sources

We further confirmed this preferential binding and the strong association between RUNX1 and p300 by performing manual ChIP and ChIP-sequencing experiments after knockdown of RUNX1/ETO.

2) Further, the result of ChIP-ChIP studies in the paper need to be verified with manual ChIP, and in all ChIP studies proper controls such as untagged strains need to be used and demonstrated.

However, due to the expected low signals of Set1 at certain loci, we have now performed additional manual ChIP experiments to verify Set1 localization.

Manual ChIP confirmation on the randomly selected putative FXR target genes from lipid metabolism category was achieved by quantitative PCR (qPCR) method [ 26].

Because we performed ChIP-chip experiment of Set1 using a FLAG antibody (Sigma, M2) against an epitope tagged Set1, we now include in our manual ChIP verification of Set1 targets a control ChIP experiment against an untagged strain.

We therefore measured binding of RUNX1, FLI-1 and SCL/TAL1 before and after RUNX1 withdrawal and at binding sites known to change binding patterns dependent on RUNX1 by manual ChIP to be able to perform accurate quantification.

The experimental design divided the movement process into the main causes of stress, comparing movement without anaesthesia (manual detachment or chipping), anaesthesia without movement and anaesthesia with subsequent movement.

For STAT6, we have tested a total of 25 regions at various levels of read enrichment selected on the basis of manual inspection to verify the ChIP-seq results at 4 h (Additional file 1).

The presented method allows the immobilization of different biomolecules on the ready-to-use reservoir covered chip without needs of manual biomolecule deposition.

Messenger RNA, cRNA synthesis and labelling reactions were performed independently for each replicate following the recommendations of the Gene Chip® Expression Analysis technical manual (Affymetrix, Santa Clara, CA).

The MOE430A and B chips were hybridized to the fragmented cRNA, stained and washed according to the recommendations of the Gene Chip® Expression Analysis technical manual (Affymetrix, Santa Clara, CA) in the Purdue University Genomics Core facility.