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Manual cell counts (hemocytometer) and cytospins/differentials were completed with fresh BAL fluid.
Cell number was determined by manual cell counting using a microscope with a counting chamber.
Seitz et al. ([1990]) determined individual biomass concentrations of an anaerobic coculture by phase-contrast microscopy assisted manual cell counting.
Seitz et al. ([1990]) made the first attempt to determine individual biomass concentrations in methanogenic microbial cocultures by manual cell counting.
Several technologies have been used, such as: FACS (Fluorescence-activated cell sorting), MACS (Magnetic-activated cell sorting), LCM (Laser capture microdissection), manual cell picking and microfluidics.
The manual cell seeding process required the removal of the lid of the cryostage when a cooled copper rod touches the supercooled sample.
The SMC adhesion, viability and proliferation were determined by MTT assay and manual cell counting at day 1 and day 3 of cell incubation.
Mean percent injected dose per cell was determined by dividing the huA33 uptake (%ID/g), calculated using the autoradiographic images, by the total number of cells in each field, determined by manual cell counting in digitized high-power H&E images.
Viability was determined by MTT assay or manual cell counting by trypan blue exclusion assay.
Viability was determined by manual cell counts and cytospin preparations made for analysis of morphology.
Cell differentials were determined from manual cell counts of stained cytospin preparations.
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