Strategies for enrichment have included manual dissection of beating areas [10], [11], Percoll® density gradient sedimentation [7], [12], and fluorescence activated cell sorting (FACS) of cells based on expression of a fluorescent reporter protein from cardiomyocyte gene promoters [11], [13].
In order to validate the manual counts, the beating of the cells was recorded on sVHS tapes through a phase contrast inverted microscope connected to a video camera.
And the automatic's fuel economy ratings, 25 m.p.g. in the city and 34 on the highway, beat the manual's 22/30.
Finally, a single research investigator followed the SuperECG Manual (Penn State University 2008c) to perform beat-to-beat ECG analysis to calculate ECG parameters.
The tool allows representation and viewing of the data, interaction with the data globally and locally at different resolutions, examining data at any point, manual adjustment of heart-beat fiducial points, and manual and automatic editing of annotations.
This may be achieved by manual dissection of areas of beating cardiomyocytes or by using fluorescence-activated cell sorting with specific dyes to select out a high purity of cardiomyocytes.
But the report (see below) also offers an inadvertent manual for weary riders trying to beat the crowds.
For CHF subjects, this combination of automated and manual preprocessing eliminated most of the premature beats and the resultant RR intervals were highly regular with only sporadic ectopic beats (undetected premature beats) and other abnormal beats such as post-ectopic compensatory pauses.
I learned some time-beating gestures from Max Rudolf's conducting manual.
(Ferreira also claims the speed with which it pulls in the data beats Flipboard, but since the interface requires manual refreshes, this is hard to confirm).
