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All sequences were aligned using the integrated aligner tool and the fast aligner option, followed by manual alignment of the sequences to closely related sequences in the ARB database.
The T. thermophila and S. cerevisiae TEN-domains were aligned to the human TEN-domain using Psi-blast, followed by manual alignment of the remaining species.
Figure 4 Graphical user interface for manual alignment of the analysis signal.
However, mismatches did occur, and additional manual alignment of SPECT and CT images was performed in all cases.
There is not yet a satisfactory technological solution for 2 main problems in the design of a sectionable cassette for biopsy specimens and small specimens: maintaining the orientation of the sample at the end of grossing and substitute fine skill manual alignment of the sample at the surface of the block for microtomy.
Step 1: Manual alignment of the Temecula-1 and M23 genomes.
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SISYPHUS is a database that contains a collection of hand-curated manual alignments of proteins with non-trivial relationships, several of which have circular permutations.
Manual alignments of human and yeast TORs to template were exported in FASTA format to the SwissModel server (24).
Our manual alignments of sequences allowed us to estimate 5.2% P/A (including P/Ad) and 4.7% AL genes between two rice genomes.
The pitch of the α-helix 4/5 allowed precise manual alignment [39] of the calcium-free EF-hand pair models independently of the position of the other α-helices of the calcium-bound lobes of the HsAPC-1 RD structure.
In the process of this manual alignment, we removed hundreds of obvious pseudogenes from outgroup sequences.
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