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To identify genes regulated in an EGFR-dependent manner, we treated EGFR-mutant NSCLC cells with gefitinib.
In order to find whether PC3 and PC3-CXCR4.5 cellsecretete CCL20 in a CXCR4/CXCL12 dependent manner, we treated the cells with antibodies to CXCL12 and PTX.
In order to understand whether TGF-β can play a role in controlling cell morphology in a paracrine manner, we treated low-TGF-β, low-contractility cells (A375P) with conditioned media from high-TGF-β, high-contractility cells (A375M2).
To further confirm that Nox4 is modulated in a TGF- β/SMAD3-specific manner, we treated MDA-MB-231 with either TGFBR1- (616451) or SMAD3 (SIS3 -specific inhibitorSIS3 -specific(DMSO) followed by TGF- β stinhibitors
To test if dRif1 also responds to damaged DNA in a similar manner, we treated S2 cells with hydroxy urea and aphidicolin and asked if dRif1 relocalized to halted replication forks.
In order to address whether GATA3 stability was regulated in a 26S proteasome-dependent manner, we treated cells with CHX in combination with the 26S proteasome pharmacological inhibitor bortezomib (Btz).
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We typically also treat diseases in an additive manner; we treat each disease in a disease complex as an independent phenomenon instead of addressing connections and interactions between different pathologies in the same individual (corresponding to aspect 4 defined above).
Since some survey rounds accounted for civil partnership status in various manners, we treated all civil partnerships as if they were marriages.
To test whether ERK kinase promotes FBW7 degradation in a phosphorylation-dependent manner, we first treated cells with MG132 to block the proteasome pathway.
In this manner, we can treat PCs as compositions containing some fragments of all initial features or, in the other words, a mixture of initial features in different proportions.
To determine whether GATA3 protein turnover is regulated in a similar, proteasome-dependent manner in breast cancer, we treated cells with cycloheximide and a proteasome inhibitor, MG132.
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CEO of Professional Science Editing for Scientists @ prosciediting.com