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In an effort to evaluate serum from autoimmune and immunodeficient patients for Abs against cytokines, chemokines, and growth factors in a high-throughput and unbiased manner, we constructed a multiplex protein microarray for detection of serum factor-binding Abs and used the microarray to detect autoantibody targets in SLE.
Further, to examine whether the miRsh-sgRNA cassette can produce gene mutation in a cell type-specific manner, we constructed an expression vector using the embryonic stem cell-specific mouse Oct4 gene promoter (mOct4P) to express the miRsh-sgp53 cassette.
In this manner, we constructed four different data sets containing 1, 3, 5 and 7 different copies of chr22.
To confirm that DArT markers were inherited in a Mendelian manner, we constructed a linkage map for a cross between T. monococcum accessions MDR308 and MDR002 [ 8].
To confirm whether Ser is phosphorylated in a calcium-dependent manner, we constructed an AtTKL1 variant where this residue was mutated to alanine (AtTKLS428A).
To identify regions of the phylogeny of Triticeae that have not evolved in a tree-like manner, we constructed a multigenic network summarizing information conveyed by individual gene trees.
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Since (mathfrak {C}) is finite, by this manner, we can construct a scaling invariant ((mathfrak {C'},mathrm {d'})).
In addition, as a further check to ensure that evaluating only a subset of those randomized is not skewing the results in any unexpected manner, we will construct a composite binary secondary outcome that will be evaluated alongside the primary outcome on all randomized women who get pregnant.
To verify that ExoS54-Cre injection occurs in a type III specific manner, we also expressed this construct in a type III defective strain, PAK- JΔpopD [21], and saw no intracellular Cre recombinase upon TE26 infection (Fig. 2C).
In a similar manner, the constructed primer sets amplified DNA from Ostertagia lyrata, Haemonchus contortus, Trichostrongylus axei, Cooperia surnabada and Cooperia punctata.
While the faces in the work are, in fact, in the news, it is only in a very superficial manner that is constructed in a randomized, albeit programmed way.
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