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In addition to the first retrieval of bone at the osteotomy site as described above, a second bone and bone marrow biopsy was recovered from the immediate, lateral metaphysis in a similar manner, to test for the migration of infection to this region.
Gene expression analyzes were performed using 3 arrays, representing 3 biological replicates with one replicate done in a dye-swap manner to test for dye-based bias.
Because of high processing speed (<1 min for complete data analyses), the software can be used in an interactive manner to test many different parameter sets.
Problems with subject recruitment underscore the need to create an infrastructure for clinical research and to design pragmatic trials that can be implemented in a timely manner to test potential treatments in patients with rare diseases like FSGS.
Neuroimaging researchers need to take a page from the sciences of climatology and geology, from economists and others who cannot always manipulate the environment in a precisely controlled manner to test their models.
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In effect, this implies that to adequately test species hypotheses, it is necessary to sample densely-enough, and adaptively, in a manner designed to test observed discontinuities between putative species [ 40].
Capability to predict these sequences in a patient makes DBNs a promising prognostic tool for physicians in order to treat patients in a timely manner, or to test a treatment efficacy.
These data and our present results showing that Wip1 activation was induced by Nut and low dose of RITA (Supplementary Figures S6D and E and Figure 4e, respectively), but was repressed by 1 μM RITA in the JNK-dependent manner, prompted us to test how inhibition of Wip1 contributes to p53 activity.
Thus, while further validation for the 5C-CPT as a test of vigilance is still required, the authors believe that the 5C-CPT provides an opportunity to assess vigilance in mice, in a manner relevant to testing conducted in psychiatric populations.
In a similar manner, ELISA was undertaken to test serum testosterone levels according to the maker's instructions.
Immobilization in this manner also allowed us to test the sparingly soluble α2 design by limiting self-aggregation.
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