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In addition, compound Ia also strongly inhibited in a dose-dependent manner the nuclear translocation of a GFP form of p65 (a subunit of NF-κB) by TNF-α in HeLa cells (Fig. 4C).
Specifically, by altering spatial arrangement, we have shown for the first time that altering colony diameter, the distance between colonies, and the degree of clustering of a culture can modulate in a predictive manner the nuclear levels of pStat3 in mESCs (Fig. 5).
In this manner, the nuclear scaffold/matrix is dynamically modified during the cell cycle to serve a continuously changing role.
Furthermore, 5-HT induced in a time-dependent manner the nuclear levels of phospho-Smad3, the typical intracellular mediator of TGF-β signalling.
In the airway epithelium, recombinant human IL-1β and recombinant murine TNF-α induced, in a time-dependent manner, the nuclear translocation of HIF-1α.
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On the other hand, the use of war as an instrument of policy was calculated in an unprecedented manner by the nuclear superpowers, the United States and the Soviet Union.
The phosphorylation levels of Foxo1 reduced in a time-dependent manner, whereas the nuclear accumulation of Foxo1 increased (Fig. 4A).
An attractive idea is that tRNA import is driven by the ATPase cycle of the Hsp70 in a similar manner to the nuclear import driven by the Ran GTPase cycle.
Also, as expected, a significant down regulation of NF-κB subunits (p65, p55 and p50) in a timely manner in the nuclear fraction suggesting that PDBD effectively inhibits NF-κB activation.
Each battleship was a signal of national power and prestige, in a manner similar to the nuclear weapons of today.
On the basis of these and similar comparisons, many authors have reached the conclusion that mtDNA evolves in a manner distinct from the nuclear genome.
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