Transcriptional regulation responds to a stressor in a sensitive manner and continuous dysregulation of transcript expression can be expected to manifest itself earlier than detrimental changes on the protein level.
This suggests that some antibodies are staining the cells in an unspecific manner, and that assessment of transcript presence by RNA-seq can provide guidance for validation of the corresponding antibodies.
XBP1, encoding a stress-induced transcriptional repressor, was found to be regulated in an oxygen-dependent manner: the level of transcripts of this gene was lowest in anaerobic, highest in the fully aerobic and on a similar, intermediate level in the intermediate oxygen conditions.
Although the Mac promoter is expected to drive constitutive expression of E1 gene in transgenic plants, the gene was expressed in a somewhat organ-specific manner, with the highest levels of transcript being detected in the leaf and the lowest levels in the root.
We chose a two-step adaptor ligation-based directional RNA-seq protocol, which provides transcript coverage in a strand-specific manner and permits a precise mapping of transcript 3′ ends [ 21].
Here, the functions of 86 lincRNAs acting as 56 miRNA decoys were predicted, and it was found that they can inhibit miRNA functions in a spatial- or temporal-specific manner, thus contributing to the regulation of transcript complexity in maize.
However, RNA-Seq suffers from an intrinsic bias that affects the quantification of transcript expression in a length-dependent manner.
End synopsis of transcript.
In turn, it affects accumulation of rhythmic transcripts in a time-of-day dependent manner: Transcripts peaking in the evening like AtGRP7 itself mainly are expressed at reduced levels in AtGRP7-ox plants whereas transcripts peaking in the morning mainly are expressed at elevated levels.
We found that there was not a systematic manner by which expression levels of transcripts related to their protein products.
