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A major strength of the TRE reporter approach is that it is now possible to read out TR-dependent T3-sensitive reporter activity in a cell specific manner during development.
The genes encoding the Drosophila insulin-like peptides are independently transcriptionally regulated in response to nutrition, as well as in a tissue- and stage-specific manner during development [3], [17].
In vitro study showed that disruption of exon 9 recognition from its 3' splice site by Fox and ESRP through UGCAUG and ISE/ISS-3 promoted switching to exon 8. Remaining question is whether the expression of Fox and ESRP coincides with the expression of exon 8 form in tissue-specific manner during development in vivo.
Another example is the fact that miRNAs are often expressed in a strict tissue-specific manner during development; for instance, miR-124a is expressed only in brain and spinal cord of vertebrates and ventral nerve cord of insects [40], [41], [42].
Are different enzymes recruited by TR in a gene- and tissue-specific manner during development?
This fine tuning ensures gene regulation in a cell specific and spatio-temporal manner during development.
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Biological pathways are dynamic and behave only in certain ways and controlled manners during development and in response to external factors [17], [22], [32].
A peak in mRNA levels was observed at hatch (Stage 32) after which levels rose in a gradual manner during larval development (Stages 33, 34, 35, and 36) to reach maximal values at first feeding (Stage 37).
However, it is generally accepted that the cell number of the involved bacteria changes in a species-dependent manner during disease development.
In order to generate the correct number of cells in the proper places, the balance between cell proliferation and differentiation must be regulated in a very precise spatio-temporal manner during brain development.
Risk assessment was performed in an iterative manner during the development process.
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conditions during development
characteristics during development
processes during development
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manner during catalysis
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manner during embryogenesis
manner during summer
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