Moreover, mutations may affect protein function in a cell-specific manner, possibly by interacting with regulatory proteins or complexes that are cell-type specific.
Here, an intrinsically disordered p27 binds to the binary cyclin A Cdk2 complex in a stepwise manner, first by interacting with a groove of the cyclin A and then via binding to the hydrophobic interaction sites Cdk2 had originally hidden from interaction.
(3) Recent work has demonstrated that the NCOA4 transcriptional coactivator also binds Mcm7 in a relevant manner to inhibit DNA replication by interacting with the CMG complex, blocking its helicase activity, and negatively regulating the activation of origins of replication [ 100].
In this manner, it acts as a decoy receptor by interacting with the ligand and type II receptors, and prevents the ligands' access to a functional receptor complex, thereby blocking TGFβ signaling (Figure S2A).
Moreover, all carbamates inhibited in vitro tubulin polymerization, in a similar manner to that of CA-4 and AmCA-4 by interacting with the colchicine binding site in tubulin.
Whether this secreted Klotho product can modulate lifespan by interacting with FGF signalling in a FGF23 independent manner has not been investigated.
