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RmS-15 delayed the clotting of plasma in a dose-dependent manner as determined in a recalcification time assay.
Exposure of both cancer cells to zerumbone or cisplatin demonstrated growth inhibition at a dose-dependent manner as determined by the MTT (3-[4,5-dimethylthiazol-2-yl]-2,Sdiphenyltetrazolium bromide) reduction assay.
Initially we confirmed that TNFα treatment increases optineurin gene expression in A549 cells in a time-dependent manner as determined by real time RT-PCR analysis (Fig. 1A).
NSC23925 was shown to increase intracellular accumulation of calcein AM in these cell lines in a dose-dependent manner as determined by both image analysis (Fig. 6A and 6C) and by microplate spectrofluorometer analysis (Fig. 6B and 6D).
Cells treated with MDR1 siRNA were shown to decrease calcein AM efflux in a dose-dependent manner as determined by image analysis, and confirmed by microplate spectrofluorometer analysis (Fig. 5).
Our previous study using MCF-7 breast cancer cells showed that HRG rapidly up-regulated hundreds of transcripts in a ligand dose-dependent manner, as determined by employing expression arrays using the Affymetrix U133A 2.0 chip that mounts protein-coding and non-protein coding regions of ca. 18,400 transcripts [4].
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(A) Ursolic acid inhibited proliferation of the four CRC cell lines in dose- and time-dependent manners as determined by MTT assay.
IL-23 induced the mRNA and protein expression of Th17-type cytokines in dose- and time-dependent manners as determined by real-time PCR and ELISA assays (Figs. 1B, 1C and S1).
PPM supply specifically increased the phosphorylation of ERK1/2 and p38 MAPK by 5 min and 2 min respectively (Figure 2B and 2C) in a dose-dependent manner (Figure 2D) as determined by immunoblotting.
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This increases the complexity of data interpretation, but it is possible to quantitate the product ions of specific precursors in a pseudomultiple reaction monitoring (pMRM), or all-reactions monitoring (ARM), manner, as well as to determine identity in a single run.
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