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Enzymes and reagents for DNA manipulations were purchased from TAKARA.
Enzymes and kits for DNA manipulations were purchased from UAB Thermo Fisher Scientific Balticss" Vilnius, Lithuania).
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Japanese flounder (Paralichthys olivaceus) (average 11.2 g) were purchased from a local fish farm and acclimatized in the laboratory for two weeks before experimental manipulation.
were purchased.
Construction of plasmids and other genetic manipulations were performed according to standard procedures [ 74] using enzymes and kits purchased from UAB Thermo Fisher Scientific Baltics (Vilnius, Lithuania).
The manipulations were performed in dim light.
Beforehand, three key manipulations were carried out.
All manipulations were done under infrared illumination.
Standard genetic manipulations were performed throughout.
Images manipulations were performed by using Adobe Photoshop CS.
Three aperture manipulations were studied.
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