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Main effects for the experimental manipulations were not found.
This is especially significant since our genetic manipulations were not aimed at altering the levels of VEGF or its receptors, VEGFR1 and VEGFR2 expressed in the glomerular endothelium.
Manipulations were not strong or excessive during the procedure.
These manipulations were not of immediate interest to the main hypotheses of the current report and the results are therefore presented in the Supplemental Experimental Procedures.
However, contrary to such an interpretation, the effects of the Potential Harm and Yedasentience manipulations were not additive: For high hypnotizables, when yedasentience was not blocked, potential harm had no significant effect on washing time, and when potential harm was low, the blocking of yedasentience had no effect on washing time.
It is clear that Tcf7l2 is expressed in multiple tissues and therefore in the other models glucose tolerance may be affected by changes in other tissues as those manipulations were not tissue specific, unlike the Xavier et al. experiment where there is deletion in islets alone.
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Moreover, in many cases in which the activity of command-like neurons has been experimentally manipulated (Jing, 2009), the behavioral impact of the manipulations was not assessed in intact and freely moving animals.
Interactions between the genetic background and experimental manipulations are not predictable and must always be tested in order to reach conclusions whether mutation effects can be generalized.
Genetic manipulations are not possible in Giardia, which is tetraploid [66].
We show that early manipulations are not completely buffered at post-implantation stage.
If these manipulations are not successful in achieving target blood pressure other agents may be added.
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