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A number of diverse manipulations were completed under the direction of federal agency and university scientists, and abundant remote sensing imagery is available to assist in relocating the treatments and evaluating their success.
Other alignment manipulations were completed in BioEdit.
All data manipulations were completed using SAS statistical package, version 9.2, (SAS Institute, Inc., Cary, North Carolina).
All manipulations were completed by 18 30 h and the investigator had left the animal room before the night phase began.
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The use of only one recombinase system to mutate multiple genes limits the scope of cancer research because all genetic manipulations are completed at the same time.
Vessel manipulation was completed in oxygenated Krebs-Ringer bicarbonate (KRB) buffer maintained at 37°C.
Data manipulations and analyses were completed in SAS 9.0 (SAS Institute, Cary, North Carolina USA).
After PICU admission, the first recording was started immediately after all necessary manipulations (examinations, laboratory specimens) were completed.
Data manipulation and statistical procedures were completed using SAS statistical software (ver. 9.1.3, SAS Institute, Cary, NC, USA) and SigmaPlot (ver. 11.1, Systat Software, Inc., San Jose, CA).
No other tasks were completed between the manipulation and AMT testing.
Sixty trials were completed once at baseline and once following the devaluation manipulation (see below).
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