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All solution preparation and manipulation was conducted in a low-oxygen, nitrogen-filled glove bag in order to minimize oxidation.
The F0 manipulation was conducted in with PRAAT (version 5.4; cf. [67], see also http://www.fon.hum.uva.nl/praat/) in two successive steps.
A snow manipulation was conducted in a Tibetan spruce forest to explore the effects of snow absence on winter soil nitrogen dynamics by shelter method.
No experimental manipulation was conducted during this observational period.
The Q photoproduct was produced as described above, and, because it is light sensitive, all manipulation was conducted under dim red light.
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All the statistical analysis and graph manipulation were conducted using R language version 3.2.1 [28].
Genomic analysis and manipulation were conducted in the Galaxy environment [ 28, 29].
During this process, no twirling lifting and thrusting manipulation is conducted.
There are two relevant situations, firstly, when the manipulation is conducted because a suitable dosage form is not available.
It is also important to note that due to the nature of learning manipulation, a series of practise manipulations were conducted which may alter the stiffness properties of the spine being manipulated and therefore alter the kinematic profile of the technique.
DNA manipulations were conducted according to standard procedures (Sambrook and Russell 2001).
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