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The EMBOSS package [ 58] was used for routine manipulation of sequences.
Here the authors just show that manipulation of sequences flanking the 3' end of the clr4 gene prevents heterochromatin assembly over clr4 in mst1 epe1 double null cells.
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Resulting R data structures allow manipulation of sequence, quality, alignment and other information.
Manipulation of sequence data were performed by the SISEQ software version 1.59 (Sato 2000).
Although representing each base with the first letter of its chemical name provides a convenient mnemonic for generating and interpreting genetic sequences, the Roman characters do little to support the visualization or manipulation of sequence data.
Within low-throughput genomic data many errors can be characterized simply using the Sequence Alignment/Map (SAM) specification [ 1] and tools are available for reading and manipulation of sequence files.
Manipulation of sequence intervals was carried out with IRanges (Pages et al).. Principal component analysis (PCA) was performed using the R package prcomp (R Development Core Team 2012), on SNVs without scaling variables to avoid overcontribution of rare variants (McVean 2009).
Most manipulations of sequences do not require that they be predeterminate, and can be performed on these more general free choice sequences.
It is assumed that a clear physical separation of the first cleavage position and the miRNA sequence provides a high flexibility for manipulation of amiR sequences [ 19, 21].
SEQUENCE-SPECIFIC nucleases are a critical tool for manipulation of DNA sequences.
Manipulation of genomic sequences facilitates the identification and characterization of key genetic determinants in the investigation of biological processes.
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