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This paper reports the preparation of defect-free asymmetric membranes through the manipulation of membrane fabrication variables, including polymer concentration, solvent ratio (ratio of less volatile solvent to more volatile solvent), forced-convective evaporation time and casting shear rate.
Chelation of calcium, inhibition of calcium channels, or manipulation of membrane fluidity, did not significantly alter Lp, whereas direct manipulation of the cytoskeleton via specific chemical reagents, or indirectly, through the bacterial elicitor Harpin or activation of phospholipase D, was effective.
10.7554/eLife.08009.011 Figure 5. Manipulation of membrane cholesterol content affects inflammatory responses.
This work illustrates how manipulation of membrane composition can be used as regulatory mechanism to control metabolic pathways.
Lpcat3-deficient mouse models therefore provide an unprecedented opportunity to study the physiological and pathophysiological consequences of manipulation of membrane phospholipid composition in vivo.
Pharmacologic manipulation of membrane order by adding 7-ketocholesterol and cholesterol into the culture media, which has been shown to reduce lipid order, inhibits CD4 T-cell proliferation and IL-2 production.
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We found that the in-plane orientation of a single nanomembrane can be controlled about a single point of rotation, by harnessing the deleterious scattering force that otherwise precludes direct manipulation of membranes without an attached bead.
Manipulation of membranes and of their constitutive core, lipid bilayers, is necessary to engineer systems toward applications.
Patel, T.N., Park, A-H.A. and Banta, S. (2014) "Genetic manipulation of outer membrane permeability: Generating porous heterogeneous catalyst analogs in Escherichia coli" ACS Synthetic Biology 3(12) 848-854.
At exterior concentrations of tens of nanomolar, most dye in energized mitochondria is quenched, but cellular fluorescence typically changes in response to manipulation of cytoplasmic membrane potential.
In fact, manipulation of the membrane mediators of HH signaling to reduce HH signaling leads to an increase of angiogenesis with low HH levels, but not with complete inhibition.
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