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Beyond the manipulation of bacterial genomes, the technology has recently emerged as a tool for manipulating bacterial artificial chromosomes (BACs) in various applications.
This recombinational engineering technology has been termed "recombineering" and offers a new paradigm for the genetic manipulation of bacterial chromosomes, which is far more efficient than the classical use of nonreplicating integration vectors for gene replacement.
The results describe a novel strategy for cloning and manipulation of bacterial secretion system gene clusters and provide a foundation for future studies to develop the beneficial uses of cloned type III secretion systems.
Direct manipulation of bacterial chromosomes by recombination-based techniques has become increasingly important for both cognitive and applied research.
Genetic tools for the manipulation of bacterial genomes allow for in-depth mechanistic studies of metabolism, physiology, interspecies and host-pathogen interactions.
This work establishes a basic synthetic biology tool for gene regulation between bacterial species that could be elaborated for more complex manipulation of bacterial populations in future applications.
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There were other genetic manipulations of bacterial AroA to reduce its affinity to glyphosate and conferred transgenic plants for glyphosate-tolerance [10], [14].
This indicates that niche complementarity (both positive and negative effects) was also likely to be driving the responses, and similar responses have been seen in manipulations of bacterial species richness [25].
Bacteria, in particular E. coli cells, constitute a perfect toolbox for glycoengineering, as they tolerate the incorporation and manipulation of foreign bacterial glycosylation pathways.
No other components of the immune system were, however, found to be affected by manipulation of feather bacterial load.
These methods have enabled large-scale manipulations of the bacterial genome including deletion, inversion, and insertion of DNA.
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