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Standard DNA manipulation methods were used [ 75].
General DNA manipulation methods were performed using standard techniques [ 83].
The manipulation methods were similar in the two groups.
The isolation of plasmids and common DNA manipulation methods were carried out as described previously [ 25].
The isolation of total DNA and plasmids, as well as common DNA manipulation methods were performed as described by Sambrook and Russell (2001) [ 75].
Similar(55)
Multimodal, parallel integration of microfluidics with active sorting and manipulation methods is a promising approach to overcoming these limitations.
While these approaches have been somewhat successful, the classic manipulation methods are not easily regulated and can be laborious.
The major inconvenience of magnetic force-based sorting and manipulation methods is an almost compulsory use of beads, which might interfere with cell functioning and further downstream analyses.
Each of the adaptamer-directed genome manipulation methods is predicated on the fact that recombination between two DNAs is enhanced by increasing the length of homology.
Single-cell analysis and manipulation methods are becoming increasingly important for fundamental research, as well as practical applications, such as in vitro fertilization (IVF) and pharmaceutical research and development.
Despite the fact that the genetic modification and agronomic manipulation methods are widely used to improve the nutritional value of plants, the inadequate public acceptance and soil specificity of genetically modified food are still the challenges (Martínez-Ballesta et al. 2008).
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