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Intuitive feature manipulation methods and grid-and-snap modes have been designed to support solid modelling in the MR environment.
Further work should be done by taking multiple factors into consideration, such as more complex geological objects, comprehensive modeling, manipulation methods, and visualization.
Bacillus subtilis is the most characterized gram-positive bacterium that has significant attributes, such as growing well on cheap carbon sources, possessing clear inherited backgrounds, having mature genetic manipulation methods, and exhibiting robustness in large-scale fermentations.
The RNA manipulation methods and microarray design have been previously described in detail [ 61].
Escherichia coli retains its dominant position as the first choice of host for the high-throughput production of proteins of therapeutic or commercial interest because of rapid biomass accumulation, well-established genetic manipulation methods and simple process scale-up [ 1, 2].
However, the availability of sophisticated gene manipulation methods and recent elucidation of the genome sequence of H. jecorina [ 9] raised the possibility of introducing molecular genetic methods into such improvement programmes, for example, by manipulating inducer forming pathways, signalling cascades and/or activation of transcription of the cellulase genes.
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The traction method (Hippocrates, Stimson), the leverage method (Kocher, Milch), scapula manipulation method and the last group is the combination of the prior three [ 11].
Cell sorting and manipulation methods development, and miniaturization is key to point-of-care diagnostics and therapeutics research.
As already described, various gene manipulation methods have been created and widely used by means of site-specific recombination and homologous recombination.
The major inconvenience of magnetic force-based sorting and manipulation methods is an almost compulsory use of beads, which might interfere with cell functioning and further downstream analyses.
Multimodal, parallel integration of microfluidics with active sorting and manipulation methods is a promising approach to overcoming these limitations.
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