Sentence examples for manipulation in cell from inspiring English sources

The use of sequence-specific nucleases such as TALE nucleases and the CRISPR system has recently presented an alternative method of gene manipulation in cell lines (Cermak et al. 2011; Ran et al. 2013); however, the simplicity of the homology-based targeting coupled to the predictability of the outcome and the traceability of off-target integration will support the future use of DT40 genetics.

We are using MiMICs to tag thousands of genes that allow elegant and specific genetic manipulations in cell culture (Neumüller et al. 2012) and in vivo (S. Nagarkar-Jaiswal and P.-T. Lee, personal communication).

This paper reviews the state of the art of micro-nanorobotic manipulation in single cell analysis.

The recently developed RNA-guided clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated 9 (Cas9) nuclease system has progressed to be an invaluable technology for genome manipulation in somatic cell types and germline model organisms.

The dominant functional unit of this manipulation in the cell is the interaction between DNA and protein.

This technology enables controlled genetic manipulation in Drosophila cell lines, and its simplicity offers the opportunity to study cellular phenotypes genome-wide.

Recent studies are increasingly highlighting the importance of metabolic manipulation in cancer cells and how bio-energetic and biosynthetic changes could be exploited to stop tumor cells progression.

These results suggest that this baculoviral delivery system can be engineered for site-specific genetic manipulation in hES cells.

These findings have implications for optimizing the design of custom TALEs for genetic manipulation in mammalian cells.

As gene trap system has been well-established to disrupt gene functions with selection markers/tags for subsequent functional analysis (Stanford et al., 2001), we here modified gene trap vectors and used CRISPR/Cas9 to establish a scalable tool entitled CTRL (CRISPR-mediated tagging and regulation of lncRNAs) for lncRNA tagging and expression manipulation in mammalian cells.

Feasibility of chromosomal manipulation in mammalian cells was first reported 15 years ago.