Instead, filopodia are detected by eye, and length or other spatial information are extracted by manually tracing filopodia using image manipulation software.
The events are extracted from web sources with almost no human manipulation, so the recommender is always showing new and recent events.
Following experimental manipulation, tissues were extracted and lysed in lysis buffer (150 mM NaCl, 1% NP-40, 0.5% DOC, 0.1% SDS, 1 mM EDTA, 50 mM Tris, pH 8.0, 10 mM NaF, 1 mM Na-orthovanadate, 10 mM β-glycerophosphate and one 'Complete' protease inhibitor cocktail tablet/50 ml) using a 2 ml Kontes™ Tissue grinder.
Class 2 are those that have either sustained serious traumatic injuries, are trapped in the rubble, or will require difficult, time-consuming manipulations to be extracted from the rubble and then need advanced life support maintenance until they can be transferred to a tertiary care facility for surgery and intensive care.
By observing the displacements of select points in the mechanism, the manipulation forces can be extracted in real-time to achieve force-guided manipulation of micro-scale objects.
For all genetic manipulations, total streptococcal DNA was extracted using the modified protocol of Miller et al. (1988).
Rather than using dense point clouds, the geometric skeleton (wireframe) of assemblies is extracted for further manipulation, once the as-built status is captured using the appropriate method.
Subsequently, loci containing INDELs were extracted using utilities in the vcflib VCF manipulation suite.
Data were extracted onto a Microsoft Excel 2010 spreadsheet for data manipulation.
Serum samples were extracted before intervention, right after intervention, and two hours after manipulation by venipuncture of the cephalic vein using a Vacutainer system (Becton-Dickinson, United Kingdom).
