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Subsequently, it discusses the molecular biology of α-herpesviruses, and the genetic manipulation and gene expression techniques that are utilized for the construction of virus-based tracers.
Conventional methods for DNA manipulation and gene analyses were used to characterize the gbs2018 gene variant specific for ST-17 clone and to design ST-17- and GBS-specific primers.
Nevertheless, prior to achieving the goal of host development, the prerequisite properties of a potential bacterium, i.e. genetic manipulation and gene expression efficiency, were characterized and optimized.
The SGs were immediately immersed in RNAlater to ensure the least possible manipulation and gene expression changes.
Escherichia coli DH5α and Pichia pastoris KM71 were used as hosts for DNA manipulation and gene expression.
Escherichia coli DH5α (Biomed, Beijing, China) and E. coli BL21 (Biomed) were used as host strains for DNA manipulation and gene expression, respectively.
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In parallel to such increase in drug development, an extremely active field of research is the pursuit of novel strategies to face cardiotoxicity employing new therapeutic approaches or genetic manipulation, miRNAs, and gene transfer [ 4, 163– 163].
Biogerontology has traditionally focused on demographic senescence by searching for environmental manipulations and genes that extend life span.
This is particularly important in cases that require high laser power or long duration, such as cancer photothermal therapy, tissue ablation, physical manipulation of cells, and gene manipulation.
The new vector, pLS1ROM, exhibits full structural and segregational stability and constitutes a valuable tool for genetic manipulation and regulated gene expression in S. pneumoniae.
The conservation of TOR signaling pathway has been explored in zebrafish using both pharmacological manipulation and morpholino gene knockdown [67].
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