Sequence manipulations, analyses, and alignments were performed using the LASERGENE software package (DNASTAR, www.dnastar.com).
Our pipeline was implemented using BioRuby [ 15], the NCBI Toolkit and the Entrez Programming Utilities [ 16] to facilitate data manipulation and analyses.
All data manipulation and analyses were performed using Stata software version 10.
Data manipulation and analyses were performed using SAS version 9.1.3 for Windows (SAS Institute, Cary, NC).
Data manipulation and analyses were conducted using Stata/SE version 11.0 (StataCorp, College Station, Texas, United States).
From morphometric analyses and manipulation of the growth of the underlying germline, it has been proposed that StC flatten in response to the tension generated by this growth through a "compliant" flattening process (Kolahi et al., 2009).
This will pave the way to the realization of a lab-on-a-chip for extensive analyses and manipulation, including mechanical phenotyping by optical stretching at the single cell level.
DWB oversaw the project, directed the experimental sleep manipulation, monitored subject adherence to the sleep manipulation, conducted the behavioral performance analyses, and drafted the manuscript.
It identifies p53 as a candidate in augmenting retrograde signaling leading to cardiac injury by using genetic manipulations, functional tests, ultrastructural analyses, and biochemical assays.
All immunological analyses and manipulations were performed in mice at least 18 months old.
