Those known to be genetically manipulated or extensively passaged in the lab prior to sequencing were removed from the datasets.
Using several short, well-known, extensively manipulated sequences of Kurt Cobain of Nirvana in concert, it meditates on the flamboyant suffering and often self-destructive impulses of the stereotypical young artist, especially the male one.
The physical characteristics of Sol-gels have been extensively manipulated for enzyme immobilization and these gels have attracted the attention of biotechnologists.
Moreover, because forests are valuable to humans for the products and services that they provide – especially wood – many forest ecosystems are extensively manipulated and modified by human societies, often disrupting the natural ecological patterns.
Since then, a similar strategy has been extensively manipulated in the lactococcal system by initially using sortase mediated cell wall anchoring motifs such as, LPXTG casein serine protein from L. lactis PrtP protein [ 35], S. pyogenes M6 protein [ 36] and Staphylococcus aureus protein A (SPA) anchor [ 37].
P-bodies have been shown to play important roles in regulating viral infection, whereas their interplay with bacterial pathogens, specifically intracellular bacteria that extensively manipulate host cell pathways, remains unknown.
Similar to viruses, intracellular bacterial pathogens extensively manipulate host cellular pathways to ensure their survival and replication, impacting on host cell cytoskeleton, signal transduction pathways, membrane trafficking and pro-inflammatory responses, to name a few [21], [22], [23].
The observed deregulation confirms that aphids are able to extensively manipulate plant physiology in relation to their nutrition status [ 1, 85].
This pathway has been extensively dissected in cells which have been recombinantly manipulated, and endogenously expressing cells including human ASM.
The molecular processes by which bacterial cells replicate, repair, recombine, and partition their chromosomes during exponential growth have been extensively studied, but little is known about how DNA is manipulated during stationary phase when cells are not dividing.
