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Further studies revealed that manipulated expression of ST3GAL IV led to the significant alterations of cell cycle distribution, apoptotic signal, cell proliferation and the effectiveness of imatinib treatment.
To investigate the physiological function of KLF17 on cytostatic behavior in cancer cells, we measured cell growth capacity with manipulated expression of KLF17.
In this study, we assessed expression of TP73 RNA species in patient tumor specimens and in medulloblastoma cell lines, and manipulated expression of full-length TAp73 and amino-terminal truncated ΔNp73 to assess their effects on growth.
These studies suggest that manipulated expression of NPR1 or its orthologues can create broad-spectrum resistance in crop plants, and therefore, could be a suitable strategy in improving crop plants for disease resistance [ 25].
In this study, we manipulated expression of the Mcl-1 protein in HCC cells by RNA interference and analyzed its impact on apoptosis sensitivity of HCC cells in vitro.
To investigate the role of KLF17 in TGF- β/Smad-mediated signaling, we manipulated expression of KLF17 by RNA interference (RNAi) in cancer cell lines and tested TGF- β responsive SBE-containing luciferase reporter (SBE-Luc) activities.
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Each manipulated TF modifies the expression of many downstream target genes, including other TFs, which can in turn activate or repress genes even farther downstream.
For instance, in the central nervous system of manipulated N. sylvestris differential expression of proteins (from the BIR; 2 family) involved in the inhibition of apoptosis was found (see Biron et al. 2006).
Plasticity was observed across manipulated bolting dates in the expression of all fruiting traits considered in this study (Table 3).
Upon switching on the expression of TRAIL, these manipulated cells induced strong paracrine apoptosis in human Burkitt lymphoma (BJAB) cells in vitro and in vivo.
In line with this, in the head of the ant sampled at 10 00 h during the manipulated biting, we found the differential expression of putative biogenic amine precursors and receptors.
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