Sentence examples for manipulated cell lines from inspiring English sources
Moreover, the impact of specific endocytic pathways on the cell entry and intracellular kinetics of pDNA was studied by inhibition (blockage) of either clathrin- or dynamin-mediated endocytosis by using both genetically manipulated cell lines and chemical inhibitors of endocytosis.
We used 254 gene-expression profiles collected from a variety of homogeneous B cell phenotypes by Basso et al. (2005) using the Affymetrix HG-U95A GeneChip® System; experimentally manipulated cell lines were excluded.
A classical fibroblast focus formation assay was used to assess transforming ability of manipulated cell lines.
The functions of spastin and mutated spastin have been described using genetically manipulated cell lines, fly and mouse models.
To follow up on these observed effects we profiled the expression of key angiogenic and metastatic factors in the manipulated cell lines using commercial PCR arrays.
Furthermore, the functional blood supply of tumours derived from these manipulated cell lines was investigated in vivo using non-invasive magnetic resonance imaging (MRI), and complemented with fluorescence microscopy.
Comparing growth rates of a genetically manipulated cell line with wild type usually provides valuable information on how severe the manipulation influences cellular functions globally.
To investigate how MLN4924 affects angiogenesis, we next used human umbilical vein endothelial cells (HUVECs), the most commonly used and easily manipulated cell line for phenotypic and mechanistic studies of angiogenesis.
Since GLI1 acts as a transcription factor, analysis of shRNA-manipulated ESFT cell lines by expression microarray would allow the identification of common targets of EWS/FLI1 and GLI1.
In this study, we assessed expression of TP73 RNA species in patient tumor specimens and in medulloblastoma cell lines, and manipulated expression of full-length TAp73 and amino-terminal truncated ΔNp73 to assess their effects on growth.
Thirty-nine of these genes showed a negative or positive correlation between their expression and HOXD10 expression in both HOXD10-manipulated cells and a panel of cell lines.
