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Applying this method to the sequencing of genomic quantities of DNA, however, would have been impossible without powerful computers and software to manipulate the sequence files, find the regions of overlap, and then assemble the fragment sequences into a final reconstituted whole.
Use of recombinant antibodies also provides the ability to manipulate the sequence at the genetic level, to introduce novel features that could not be gained by immunization protocols.
Hansen argued that while cDNAs are a modified form of DNA, the process used by Myriad to create them involves "letting nature manipulate" the sequence, "not the scientist manipulating" it.
The most recent assemblers use de Bruijn graphs [ 3- 10] or string graphs [ 11- 14] to model and manipulate the sequence reads.
"Add a couple nucleotides, take out one exon, manipulate the sequence a bit, and you're almost certain to fall outside of patent protection".
Overall, this strategy can be used to generate deletions, to manipulate the sequence of a particular stretch of the targeted protein or to introduce a DNA element at a particular site for further site-specific manipulations.
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BioEdit was used to manipulate the sequences (Hall 1999).
BioEdit was used to manipulate the sequences [ 62].
The program Bioperl was used to manipulate the sequences, and Matlab (MathWorks) was used to perform the analysis of the data.
The computational model and results presented here will be useful for evolving better mixing protocols (by manipulating the sequence and frequency of alternating jets).
In (Hall 1999), the ATFM in the UK was upgraded to handle increasing capacity of air traffic by developing the Central Control Function for generating and manipulating the sequence of flights inbound to a major airport complex such as Heathrow and Gatwick.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com