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To verify the regulation of OCT4 and BIRC5 expression in HCC cells, a plasmid vector expressing BIRC5-specific small hairpin RNA (BIRC5-shRNA) or OCT4-specific small hairpin RNA (OCT4-shRNA) and an adenovirus vector expressing OCT4 (Ad5-OCT4) were constructed and used to manipulate the expression of BIRC5 and OCT4 in HCC cells.
A hybrid approach involving synthetic DNA, fusion PCR, and ectopic expression has been used to genetically manipulate the expression of the D1 protein of photosystem II (PSII) in the model cyanobacterium Synechocystis sp. PCC6803.
Both overexpression and antisense suppression strategies were used to manipulate the expression of several genes encoding key enzymes in the flavonoids/isoflavonoids pathway in transgenic tobacco, lettuce, and petunia.
In conclusion, the ability to manipulate the expression of receptors in a site-specific manner can be a powerful tool.
Here, we describe the use of HIV-based lentiviral vectors to manipulate the expression of junctional signalling molecules in mouse RPE in vivo.
In addition, a virus-induced gene silencing system for transient knock-outs of gene expression has recently been established for Aquilegia [34] allowing us to manipulate the expression of all Aquilegia BEL1 and Knotted proteins.
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When the specifications are written as temporal logic formulæ, algorithms can manipulate the expressions symbolically, and progressively rewrite the original specification as the trace is being read; the pattern is violated when this rewriting process transforms the specification into a contradiction.
Manipulating the expression of membrane transporters can increase Fe concentrations in crops (Schroeder et al. 2013).
Manipulating the expression of this protein may be helpful in formulating strategies for developing preventive intervention against Salmonella infection.
The presence of complicating environments mainly manipulates the expression of strain energy added with a specific term.
Viral vectors afford the capability of genetically manipulating the expression of neurotransmitters, neuropeptides, hormones, and their receptors in specific brain sites of adult animals of any species.
More suggestions(15)
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