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Finally, we discuss progress in designing therapeutic strategies that manipulate the cellular redox environment to restrict age-associated pathology.
The introduction of microengineering tools to the biological community has provided a valuable set of techniques to manipulate the cellular environment, and to examine how cell structure affects cellular function.
Unlike the DNA-damaging agents, viruses inactivate or manipulate the cellular DNA-repair system for the viruses' benefit.
They have evolved a variety of strategies to manipulate the cellular machinery for their own benefit as well as to counteract or even to use host immune defenses.
Moreover, most characterized fungal effectors are small secreted proteins, which can manipulate the cellular processes of hosts to facilitate infection [ 5, 6].
Nevertheless, besides the importance of codon optimization, we conclude that the fasciclin GPI-anchoring signal and the lipophorin secretion signal can be used to manipulate the cellular localization of transgenically expressed proteins of interest.
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PCL biofunctionalised nanofibres are useful for manipulating the cellular microenvironment.
We find that manipulating the cellular microenvironment alters the ability of such cells to undergo cell cycle.
In this way, the objective of manipulating the cellular environment from a more restrictive state to a more permissive one might be achieved.
Moreover, manipulating the cellular environment using this novel method can maintain the proliferative potential of primary MECs, thus enabling cell cycle to be studied as an endpoint after gene transfer or gene deletion experiments.
This approach avoided potential variables introduced by overexpression of foreign DNA elements or otherwise manipulating the cellular genome.
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