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Many experimental results showed that the engineered VHs, which possessed solubility, stability, small size and consequently easier to express, purify and manipulate in vitro, were self and long-lived molecules compared to synthetic peptides.
However, we emphasize that both ArntbHLH and ArntbHLH-C/EBP were difficult to manipulate in vitro.
Although CB HSCs are relatively easier to manipulate in vitro, harbor a higher proliferative capacity and are more susceptible to gene transduction [5], [6], adult HSCs are more therapeutically relevant for human gene therapy applications.
The pRSII plasmids are easier to manipulate in vitro than their pRS predecessors and will facilitate molecular cloning and yeast plasmid construction.
Moreover, it is now increasingly feasible to obtain gene expression kinetics from in vivo and cultured follicles at defined stages and to manipulate in vitro cultured follicles.
Dendritic cells are the most effective APCs but are present in low numbers in vivo and are difficult to culture, in contrast B cells are present in large numbers, are simple to manipulate in vitro and have been demonstrated to act effectively as APCs inducing specific CTL responses in vivo (Gajewski et al, 2001).
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These biotinylated lentiviral vectors have a high affinity for streptavidin paramagnetic particles and, once captured, are easily manipulated in vitro.
Conditions under which these functions can be manipulated in vitro, as well as examples of possible loss of astrocytic function in AD, are discussed.
The basement membrane from the lens capsule of the eye is a collagen IV-rich matrix that can easily be extracted and manipulated in vitro.
Chitinolytic chlorothalonil-tolerant soil bacterium Bacillus thuringiensis SFC24 (Bt SFC24) was manipulated in vitro to express secretable form of elicitor protein harpinPss of Pseudomonas syringae pv.
Engineering the host strain and altering specificities of proteins employed for bioethanol production can be achieved by genetic engineering techniques, where the gene of interest is isolated first, manipulated in vitro and introduced back into the host organism.
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