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In zebrafish, one of the most commonly used tools to manipulate gene function is antisense morpholino oligonucleotides (MOs) that bind to mRNA.
The continued development of novel genetic tools to manipulate gene function in Drosophila has boosted the use of this organism to study in vivo biological mechanisms and to model disease (Bier 2005; Yamamoto et al. 2014).
A more complete understanding of the physiological roles of MvSrc1, MvSrc2, and MvCsk in intact Mi. vibrans cells awaits the development of experimental tools to manipulate gene function in filastereans.
This demands (i) a more precise view of genome organisation and composition, and gene structure, (ii) detailed expression profiles from multiple cell types, and developmental and physiological contexts, and (iii) the capacity to experimentally manipulate gene function.
In the era of recombinant DNA and genomics, many standard tools are available to the C. intestinalis researcher, including a well-annotated genome, methods to manipulate gene function and to create mutant strains and reporter lines, and techniques for expressing engineered DNA (Satoh, 2014).
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RNA interference (RNAi) method is a powerful tool for assessing and manipulating gene function.
Understanding and manipulating gene function in physiological conditions is a major objective for both fundamental and applied research.
Targeted modification of plant genome is key to elucidating and manipulating gene functions in plant research and biotechnology.
In our quest for understanding gene function, we commonly manipulate gene expression and compare the phenotypes of the manipulated versus control organisms.
Further understanding of what causes changes in homoeologous gene function may provide avenues to manipulate gene expression.
In order to study gene function in intact organisms, effective techniques to manipulate gene expression to achieve an over-expression or knockdown are essential.
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