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To further see whether endothelial glycosaminoglycan (GAG) binding with consecutive prostacyclin (PGI2) release is mandatory for the anticoagulant process of AT, animals were administered heparin or indomethacin either alone or in combination with AT.
Interestingly, mutational studies with the E. coli multidrug exporter MdfA also revealed that although negatively-charged amino acids play a role in substrate recognition and transport their electrostatic interaction is not mandatory for the binding process with cationic substrates [30], [31].
Although PAI-1 is dispensable for lymphangiogenesis, it is worth noting that other proteolytic systems are mandatory for this process and particularly matrix metalloproteases such as the MMP-2 whose deficiency impairs lymphangiogenesis in in vitro and in vivo models [29].
The understanding and overcoming of those undesired side effects is therefore mandatory for the implementation of optimal process parameters.
Adhesives are not mandatory for the via-first device transfer process but are used for temporary bonding of the devices on the carrier wafer or for an underfill (mechanical support).
While some nations have imposed voting as mandatory for all citizens, the process of disenfranchisement in the US appears to be tolerated and/or encouraged at least by some political elites who claim to represent us as a whole.
The draft bill seeks to grant approvals to projects on certain parameters, expedite all the approval processes mandatory for the projects to take off and rate developers on their financial strength.
Wait for the process.
Lilly's group development review (GDR) is mandatory for the approximately 500 employees who are identified through the company's talent assessment process as having executive potential.
The differential diagnosis of mandibular NHL includes other infiltrative processes such as myeloma, leukaemia and bone metastases, making biopsy mandatory for the correct histological diagnosis.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com