In this study, only a subset of amino acids was substituted at each position in TNF1, making the number of peptides in the library more manageable.
Finally, structure-based methods for sequence engineering [31], [32] may be implemented into this approach to identify replaceable positions and to limit libraries to manageable ensembles, which would allow further optimization of protein stability.
These libraries are manageable enough that the majority of sequences would be represented in each experiment.
Existing approaches, however, have proven of little utility as the primers from shortmer libraries are compromised by the necessity of constraining the library size to a manageable level.
While such advanced search functions will be profitable when large datasets are studied, the currently still manageable information in the online CAT libraries [ 11] will serve the user better if initially displayed in a broader way.
Look for small, intimate settings where he can practice his social skills in a more manageable situation, like an art class or library program.
Twenty-four genes were carefully handpicked in Yamanaka's library [4], which was more manageable than Thomson/Yu's library of 50 100 genes [5].
Using the RoToR, the library was compacted into a manageable number of plates and then printed onto plates containing 10 mM caffeine, both singly and in combination with 100 ng/ml rapamycin.
Otherwise, even the use of seven or eight screening dimensions did not suffice to identify manageable lists of putative BAC coordinates for the library with tenfold genome coverage.
This has reduced the number of plates of our 41,472 human clone library from 432 to a more manageable 108, and the rat clone library from 375 to 94.
