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Validation of SNPs with significant associations was performed using mammographic density data from two other studies.
The process of collecting mammographic density data in this study has been described previously [ 11].
We observed no statistically significant interactions between alcohol consumption and mammographic density (data not shown).
Mammographic density data are not available in the CIMBA sample to test this hypothesis explicitly.
None of the genes differentially expressed in normal breast tissue according to serum estradiol levels were independently associated with mammographic density (data not shown).
However, as mammographic density data are only available for a subset of the subjects, this variable was accounted for together with the other risk factors in a separate model.
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Of the 643 premenopausal or early perimenopausal SWAN participants with available mammographic density information, at least partial genotyping data were available for 463 (72%) women.
Women with and without mammographic density information were not found to differ significantly at the 5% level for the covariates included in the analysis models (data not shown).
Of note, no associations were observed between alcohol consumption, serum estrogen, and mammographic density phenotypes (data not shown).
We examined the role of dietary calcium and vitamin D intakes in childhood and throughout adulthood in relation to mammographic density using data from a nationally representative cohort of 1161 women followed up since their birth in 1946.
The final study group with both mammographic density and genotype data included 726 controls of 764 eligible (95%).
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