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Similar findings were observed in ErbB2-induced mammary tumours from transgenic mice (Andrechek et al, 2003).
Specific COX-2 inhibitors can prevent mammary tumours from developing in experimental animals.
To determine FAK recombination efficiency, mammary tumours from FAKflox/+ and FAKflox/− mice were analysed by PCR.
Aseptically collected mammary tumours from PyMT mice were minced and immersed in cold Dulbecco's Modified Eagle's Medium (Sigma, USA).
Mammary tumours from transgenic mice expressing activated mutants of ErbB2 also express elevated levels of total and tyrosine-phosphorylated ErbB2 and ErbB3 [ 7].
By photodynamic diagnosis using aminolevulinic acid we were able to reliably distinguish primary mammary tumours from normal mammary tissue microscopically and macroscopically in all our patients.
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Furthermore, mammary tumours derived from Neu transgenic mice also exhibit co-overexpression of endogenous EGF receptor [ 20].
To determine whether the mammary tumours developed from the luminal-epithelial compartment, we stained all P53R270H-driven and p53lox/lox-induced mammary glands and tumours for cytokeratin 8 (CK8), a luminal marker, and cytokeratin 5 (CK5), a myoepithelial or basal marker.
Similarly, when mammary tumours derived from the same donors were directly transplanted orthotopically into both the left and right 4th mammary gland of recipient mice, the subsequent tumours showed divergent IFP values.
We chose J110 cells since they are Ret+/ER+ and the primary mammary tumours resulting from their injection into fat pads spontaneously metastasize to lungs and other organs (Torres-Arzayus et al, 2010).
Mammary tumours derived from transgenic mice expressing ATX or each of the EDG-family LPA receptors showed upregulation and activation of downstream pathways associated with receptor tyrosine kinase signalling, including the PI3K/Akt, p38-MAPK and ERK/MAPK pathways (Liu et al, 2009).
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