Sentence examples for mammary tissue samples using from inspiring English sources

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ChIP assays were performed with the FFPE mice mammary tissue samples using H3K9ac, H3K14ac, and H4K8ac antibodies.

Total RNA was isolated from mammary tissue samples using Trizol (Invitrogen, CA, USA) and purified using RNeasy mini kit (Qiagen, Netherlands).

Total RNA was extracted from the mammary tissue samples using RNAlater (Invitrogen Life Technologies, Carlsbad, California, USA) according to the manufacturer's instructions.

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Mammary tissue samples were used to generate four sets of transcriptome profiles from: i) inoculated quarters 6 h post infection; ii) inoculated quarters 24 h post infection; iii) noninfected quarters neighboring the inoculated quarters 24 h post infection; and iv) quarters of untreated healthy control animals.

The data for the M6 mouse breast cancer cell line, other C3(1)/Tag mouse mammary tumors and normal mammary tissue samples (FVB background) were generated using Affymetrix MOE430A arrays and processed with RMA and quantile normalization [ 15].

Mammary tissue samples were also extracted and purified using a method previously described [ 22] with minor modifications.

Quantitative RT-PCR (qRT-PCR) was used to measure the expression patterns of selected genes in mammary tissue samples taken from each animal 16 h after the S. aureus infusion (Fig. 1).

Finally, we used TUNEL staining to determine if any of our estrous cycle staged mammary tissue samples show high levels of apoptosis as occurs during mammary gland involution.

Given the observed estrous cycle dependant changes in expression of PAD2 in canine mammary tissue samples, we decided to directly test the hypothesis that PAD2 expression is regulated by specific hormones and/or growth factors in mammary epithelial cells.

Serum and mammary tissue samples were collected at study end.

Altogether, 56 mammary neoplasms and 28 normal mammary tissue samples were collected from these patients.

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