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Mammary composition was not evaluated in our study, but it may have affected mammary metabolic output.
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Our data demonstrate that reproductive state alters mammary ECM composition, which influences tumor cell metastatic potential.
These observations suggest that changes in mammary ECM composition with tamoxifen treatment could provide a plausible mechanism by which macrophage number is reduced in the mammary glands of tamoxifen-treated rats.
As confirmed by Western blot analyses, prominent differences between control and tamoxifen mammary ECM composition were the presence of high levels of intact fibrillar collagen in mammary tissue of tamoxifen-treated rats and the presence of partially proteolysed collagen and elevated levels of fibronectin in control mammary glands.
We investigated the consequences of high-fat diet (HFD) and AHR ligands on body composition, mammary development, and hepatic P450 expression.
No differences in mammary parenchymal mass or composition, with or without adjusting for empty body weight, were detected.
To determine the effect of the ECM composition on mammary cell proliferation, we tested proliferation of MCF10A cells and various SCPs on FN-coated polyacrylamide gels.
These phases are highly correlated with changes in milk composition and mammary gland gene expression [ 10, 13, 19].
Interestingly a tissue specific knockout of PPARγ altered the lipid composition of mammary gland secretions with deleterious effects on neonatal mice [ 43].
Flow cytometry was used to analyse the cellular composition of mammary glands at all eight time points up to mid-gestation [ 44].
Three broad phases can be recognised, during which the size of the mammary gland and the composition of the milk change.
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