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Fifty-seven (83%) of the malignant specimens were from patients with advanced cancer stage (⩾T3=organ and/or large vascular structure invasion).
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The correlation coefficient (r-value) for fresh and paraffin-embedded tissue ploidy analysis in the malignant specimens was 0.91.
Interestingly, a higher number of malignant specimens was positive for both receptors relative to normal ovary (97.1% and 14.3% of malignant EOC were FZD1- and FZD5-positive, whereas only 54.5% and 8.7% of the normal ovarian tissues were positive respectively).
Significant differences in TMPRSS4 gene expression between lung adenocarcinomas and squamous carcinomas or between the tumour and non-malignant specimens were analysed by unpaired two-tailed Student's t-test.
First, 12 placentas, 38 moles, and 19 malignant trophoblast specimens were compared.
The LINE-1 methylation pattern from first trimester placenta, hydatidiform mole, and malignant trophoblast specimens were compared.
After the prestain image cube was created, both normal and malignant tissue specimens were incubated topically with 2-NBDG at 37°C for 20 minutes in the dark.
Archival formalin-fixed normal and malignant breast specimens were investigated by histology, in situ PCR with confirmation of the findings by standard PCR and sequencing of the products, plus immunohistochemistry to identify HPV E6 oncoproteins.
The pre-malignant polyp specimens were tested for B cell surface phenotype λ and κ chains, CD79, CD20, CD21 and CD23 using an immunoperoxidase method.
The expression levels of the two normal ovaries and two non-malignant pathological specimens were averaged and the tumor expression data are presented relative to this average (hereafter referred to as non-malignant control).
Corresponding pairs of malignant and non-malignant renal tissue specimens were obtained from 112 ccRCC patients without distant metastasis who underwent tumour nephrectomy.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com