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The CDR3 sequences of the expanded V-J pairs were distinct in each malignant individual, even for pairing of TRBV7-3 with TRBJ2-2 that showed increased usage in both cases.
We chose the V-J pairing events that are greatly expanded in either malignant individual (greater than 5% of total population) compared to normal controls (less than 0.01% of total population in both normal samples) for CDR3 sequence analysis.
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Together, these data show that in malignant cells, individual ZNF genes and entire ZNF gene clusters across different chromosomes are silenced by spreading of H3K9me3 and DNA hypermethylation.
So while our pooled samples suggested positive correlation of differential expression of all SAS pairs between normal and malignant settings, individual assessment of a range of solid tumours and cell-lines indicated the existence of alternative patterns of differential expression.
Taking matched E-DR as indicative of malignancy, sensitivity, specificity, positive predictive value, negative predictive value and accuracy for diagnosis of individual malignant foci were 84.5, 98.5, 97, 91, and 93.3 % respectively.
We aimed to compare the level of knowledge about HPV and about the women's individual malignant disease between women with and without HPV-associated gynecologic cancer as well as the knowledge about individual malignant diseases.
A histological pattern found at the periphery of carcinomas is the presence of individual malignant cells detached from the tumour mass and staying independently within the interstitial matrix of the stroma.
The rearranged pattern is highly specific of each individual malignant cell line confirming that they are monoclonal proliferations.
Individual malignant glands showed positive staining in either the majority or none of the cells.
Table 2 shows the data for individual malignant tumours in the NF1 cohort.
So far, these approaches have not considered the high variability in the complexity of CNAs among individual malignant tumors.
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