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The males were weighed, measured, submitted to hypophysation (2.5 mg kg− 1 carp pituitary extract), and then had their semen and blood collected.
All males were weighed to the nearest 0.1 milligram using a Mettler AE 50 balance approximately one hour before the contests were conducted.
Males were weighed after the contest, euthanized by freezing and then placed in 95% ethanol for subsequent measurement of mandible length, maxilla length and maxillae span.
Subsequently, the males were weighed weekly until 40 weeks of age.
Groups of 25 adult males were weighed with an Adventurer Pro Precision balance (Ohaus).
Males were weighed on the day of PPS and daily on PNDs 41 47.
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On PND55, B-males were weighed and killed, sera were collected for measurement of testosterone, and testes and epididymides were weighed and fixed (Narotsky et al. 2013).
An Index of Condition (sensu [ 33, 48]) was calculated for every night a male was weighed.
On the day after the final moult, each male was weighed and after the male had died the tibia-patella length of both forelegs was measured under a dissecting microscope using the measuring tool of Leica IM500.
Male rats were weighed and treated intraperitoneal with 1.0, 2.0, 3.0, or 4.0 g/kg ethanol or equivolume saline (equivalent to 4.0 g/kg dosings) every 48 hours for 20 days beginning on postnatal day (PN) 30.
To obtain water content groups of 5 male flies were weighed (Sartorius, Göttingen) after anesthetizing them on ice (living wet weight) and were subsequently dried at 60°C for 24 hours.
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