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The males were then taken away from their females, to limit any influence they might have on their progeny.
The genetically modified males were then bred with normal females to produce large litters.
males were then backcrossed to CyO, dplvI cl-4/Gla females.
F1 males were then scored for mosaic or complete dumpy mutant phenotypes.
Females and males were then removed, and females were screened for infection status.
Mutagenized males were then crossed to EK females and the progeny (F1) were raised.
The males were then individually incubated with 5 virgin females during 24 h and subsequently isolated.
Similar(4)
Males are then released into the wild, where tetracycline is not available.
The sterilised males are then released by plane (roughly once a week, 100 flies per sq km) to mate with wild females.
This is because mutant males are then equally compatible with females whether they have the infection or not, and loss of Wolbachia increases fitness.
Each of the 96 males was then scored for the presence of a recombination event in the relevant interval.
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