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Following eclosion, males were removed.
Any males were removed and not included in the analysis.
After 24 hours, males were removed and hermaphrodite epa-18 nematodes were isolated to separate plates.
After two weeks of co-housing, males were removed and each female remained housed individually when raising offspring.
Males were removed when the female was visibly pregnant, and the cage monitored for the timing of birth of the litter.
After 10 days, the females' pregnancy state was estimated visually and/or by palpation of the embryos along the backbone, and the males were removed.
After 15 days males were removed and pregnant females were isolated in clean cages, and inspected twice a day for live pups.
As juveniles, these offspring were raised together in family-specific tanks and males were removed upon maturity and reared separately with untested females from their own population.
After mating for around 10 h, all males were removed from the bottle to avoid diminishing CI effects with increasing male age.
To examine the adult testes of mutant mice in more detail, urogenital systems from wild type and Mrotm1H homozygous males were removed, fixed in Bouins for 4 to 6 hours, then transferred to 70% ethanol.
After 24 hours, males were removed and hermaphrodite glp-4 nematodes were isolated to separate plates and transferred to 15°C, which is the permissive temperature for glp-4.
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