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Transverse hippocampal slices from 8 14 week old males were prepared in cutting saline (200 mM sucrose, 3 mM KCl, 1.4 mM NaH2PO4, 26 mM NaHCO3, 2 mM MgCl2, 2 mM CaCl2, 10 mM glucose) and maintained in an interface chamber containing artificial cerebrospinal fluid [61], [62].
About 500 females and 1000 males were prepared for each biological replicate.
Chromosome squashes of testes from the Anastrepha males were prepared as previously described [ 58, 59].
Ten- to 12-week-old males were prepared and treated as described below.
Pregnant B10.PL females (crossed with B10.PL males) were prepared for image acquisition between gestational days 15 and 17 of pregnancy.
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The chemical extracts from a sib male and a non-sib male were prepared from males frozen at −20°C on the day of their emergence (conserved less than 15 days).
Subsequently, castings (male) were prepared from these, using hot-melt glue.
Apparently, unlike your average Renaissance artist, no contemporary male is prepared to openly admire the naked male form.
Methods: Male rats were prepared according to the chronic constriction injury (CCI) model.
At 10 to 12 weeks of age, male mice were prepared for monitoring of EEG/EMG signals [22].
Limbs from male mice were prepared for IHC as described previously (10).
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