Additionally, 20 females and 20 males were placed on NFD and allowed to lay eggs for 3 days to create the subsequent generation.
Half the males were placed in vials in groups of four, each group with 20 female flies that were ready to mate, allowing the males to mate with multiple females.
When the sons of highly reactive males were placed with unusually nurturant mothers, this personality trait was completely prevented.
After injection, males were placed in individual cages with ample water, food, and cover.
Upon maturity, 50% of the males were placed with females and allowed to breed.
In the isolation condition, males were placed in an individual cage in a sound isolation chamber with no other birds.
7 10 day-old males were placed in vials containing 1% FD&C Blue No. 1[MacCormick]/10% sucrose/1% agar for 6 h or 24 h.
Twenty males were placed in a glass vial with a screw-on cap containing 800 µl of distilled hexane, for 60 min, to obtain whole body extracts.
Males were placed in a cage of 10×10×10 cm with walls consisting of a wire mesh (2 mm pore size).
When groups of eight Poxn-pRes; Or83b2 males were placed in a Petri dish, some of them started courting each other and formed courtship chains (Movie S4).
