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Males were injected with 200 IU hCG and then paired with individual females.
Males were injected within two minutes of each other to ensure that longevity of sperm did not confound our experiments.
Wild-type B6CBA F1 males were injected intraperitoneally every day from P12 to P25 with 10 mg of pochoxime A per kg of body weight.
40 C57BL/6 males were injected three times intraperitoneally with 60 80 mg/kg bodyweight ethyl-N-nitrosourea (ENU) with one-week intervals.
Transgenic and control males were injected with cadmium, and the histology of the testes was examined.
BALB/c or C57BL/6 males were injected intraperitoneally with 5-fluorouracil (150 mg/kg) and were killed after 4 days.
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The same procedure was used also in sperm competition treatments, with the sperm of the two males being injected on the eggs simultaneously.
From 125 days of gestation (term, 145 ± 2 d), 10 ewes carrying single fetuses of mixed sex (3 female, 7 male) were injected twice im, at 10 11 pm, with dexamethasone (2 × 12 mg, n = 5) or saline (n = 5) at 24-hour intervals.
This male was injected with 150 IU hCG.
The resident male was injected with the dye and the 'intruder' male was injected with saline (0.9% NaCl).
Each male pair was used only once and, in each replicate, only the 'resident' male was injected with the dye.
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